The project ‘dnatox’ consisted of three subprojects: (1) LC-MS of toxic compounds, (2) LC-MS of DNA, and (3) software development.
The subproject ‘LC-MS of toxic compounds’ focused on the development of liquid chromatography-mass spectrometry (LC-MS) workflows for the comprehensive identification of toxic agents. Tandem mass spectral data was used for compound identification. Reference spectra of >1000 compounds were acquired and stored in a database. A sophisticated algorithm enabled sensitive and specific library search.
The subproject ‘LC-MS of DNA’ focused on applications of LC/MS in forensic genetics. LC/MS was found to be particularly useful to genotype autosomal short tandem repeat (STR) marker. In comparison to conventional electrophoretic sizing, increased forensic discrimination was obtained due to the simultaneous differentiation of length and nucleotide variability.
In the subproject ‘software development’ tools for the automated, computer-assisted interpretation of mass spectral data were developed.
Project leader:
Institut für Gerichtliche Medizin der Medizinischen Universität Innsbruck
Prof. Dr. Richard Scheithauer, Institutsdirektor
Other Project partner:
Iplexx IT Solutions KG
Ing. Martin Pircher, Innsbruck
CEMIT Center of Excellence in Medicine and IT
Dipl.Inf. Patricia Schirmer, Innsbruck
Umweltbundesamt GmbH
Gerhard Thanner, Wien
Bundesministerium für Inneres,
Bundeskriminalamt
Dr. Reinhard Schmid, Wien
Applera Austria Handels GmbH
Dr. Karin Wihsböck, Brunn am Gebirge
Projekt leader:
Medizinische Universität Innsbruck
Institut für Gerichtliche Medizin
Müllerstrasse 44
A-6020 Innsbruck
Tel. +43.512.9003-70600
Fax. +43.512.9003-73600
Contact:
Prof. Dr. Richard Scheithauer
richard.scheithauer@i-med.ac.at
Web: www.gerichtsmedizin.at
Project management:
CEMIT Center of Excellence in Medicine and IT
Leopoldstrasse 1
A-6020 Innsbruck
Contact:
Dipl.Inf. Patricia Schirmer
Programm- und Projektmanagement
Tel. +43.512.576523-233
Fax. +43.512.576523-301
patricia.schirmer@cemit.at
Web: www.cemit.at
